Recombinant Human Anti-EBV BLLF1 Antibody (72A1) (CAT#: PABZ-138)
Recombinant Human Antibody (72A1) is capable of binding to EBV BLLF1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-EBV BLLF1 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-EBV BLLF1 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. 72A1 neutralizes EBV infection by blocking the attachment of gp350 to CR2.
Specific Inquiry
SDS-PAGE
Figure 1 Recombinant Human Anti-EBV BLLF1 Antibody (PABZ-138) in SDS-PAGE
SDS-PAGE analysis of PABZ-138 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Recombinant Human Anti-EBV BLLF1 Antibody (PABZ-138) in SEC-HPLC
The purity of PABZ-138 was greater than 95% as determined by SEC-HPLC.
ELISA
Figure 3 Recombinant Human Anti-EBV BLLF1 Antibody (PABZ-138) in ELISA
ELISA analysis of PABZ-138 was performed by coating with Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 / EBV GP350 Protein (His Tag).
The secondary antibody: HRP-anti-Human IgG
WB
Figure 4 Recombinant Human Anti-EBV BLLF1 Antibody (PABZ-138) in in WB
Western blot analysis of PABZ-138 was performed with Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 / EBV GP350 Protein (His Tag).
PABZ-138 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-anti-Human IgG (H+L)
DB
Figure 5 Recombinant Human Anti-EBV BLLF1 Antibody (PABZ-138) in DB
Dot Blot analysis of PABZ-138 was performed by coating with Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 / EBV GP350 Protein (His Tag).
The secondary antibody: HRP-anti-Human IgG
Specifications
- Immunogen
- EBV Viral envelope glycoprotein
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native EBV BLLF1
- Species Reactivity
- EBV
- Clone
- 72A1
- Applications
- IF 1:100-1:500
Block Assay-Dependent
Neut Assay-Dependent
ELISA Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody 72A1 has been reported in applications of IF, Block, Neut, ELISA. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- Viral envelope glycoprotein; Major outer envelope glycoprotein gp350; BLLF1
- Gene ID
- 3783713
- UniProt ID
- Q9QP87
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-138 | Recombinant Human Anti-EBV BLLF1 Antibody Fab Fragment (72A1) | Neut, ELISA | Fab |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABZ-138, RRID: AB_3111709)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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